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Table 1 Digital PCR Workflow

From: Impact of variance components on reliability of absolute quantification using digital PCR

Step Output Associated variation
1 Extracted DNA Inhibition, overdilution, underdilution
2 Reaction mix Pipette error, sample heterogeneity
3 Partitions Loss of partitions, unequal partition size
4 Fluorescence signal Loss of partitions, amplification efficiency
5 Estimated Misclassification, model uncertainty,
  concentration inaccurate partition size
  1. A summary of the steps in digital PCR with associated variance components.