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Table 1 Digital PCR Workflow

From: Impact of variance components on reliability of absolute quantification using digital PCR

Step

Output

Associated variation

1

Extracted DNA

Inhibition, overdilution, underdilution

2

Reaction mix

Pipette error, sample heterogeneity

3

Partitions

Loss of partitions, unequal partition size

4

Fluorescence signal

Loss of partitions, amplification efficiency

5

Estimated

Misclassification, model uncertainty,

 

concentration

inaccurate partition size

  1. A summary of the steps in digital PCR with associated variance components.
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BMC Bioinformatics

ISSN: 1471-2105

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