Figure 4

ConSeqs error profile. MT-Toolbox derived ConSeqs have fewer EPB than raw reads, and errors within ConSeqs are reduced as MT depth increases. MT-Toolbox ConSeqs generated from overlapping PE reads are the most accurate even at low MT depths. ConSeqs built from forward and reverse reads are slightly less accurate then overlapping PE ConSeqs. Raw reads of any type are the least accurate. MT-Toolbox ConSeqs generated from PE reads at depths ≤10 (88% of the data) are more accurate than those generated by LEA-Seq. EPB were calculated by averaging individual EPB of ConSeqs having the same depth. Error bars represent standard error and grow in length as depth increases due to fewer MTs having high depths (Figure 3).