Figure 1
From: A tool for design of primers for microRNA-specific quantitative RT-qPCR
Position of primers for miR-specific RT-qPCR. The sequence of the forward primer (F primer) is identical to 12 – 18 nucleotides of the microRNA and may include a tag at the 5′-end. The reverse primer (R primer) is complementary to 3 – 8 nucleotides of the microRNA, followed by 15 T residues and a tail of varying length. The 15 T’s and the tail are identical to part of the RT primer sequence.