Specific amplification of a target from a biological sample. Detection of ssc-miR-15a with specific primers in cDNA made from purified pig lung total RNA (see Additional file 3). A Amplification curves. B Melting curves. C Extrapolation of Cq as function of the log10 of the relative number of templates was a straight line (R2 = 0.99) with a slope of -3.40 (PCR efficiency = 96%) over 4 log10 dilutions of a pool of all the samples used in the experiment.