From: A tool for design of primers for microRNA-specific quantitative RT-qPCR
Parameter | Values | Score |
---|---|---|
2 nucleotides at 3′-end | 0 or 2 Wsa | 0.7 |
1 Wa | 1.0 | |
3 nucleotides at 3′-end | 0 or 3 Wsa | 0.3 |
1 or 2 Wsa | 1.0 | |
0 or 5 Wsa | 0.1 | |
5 nucleotides at 3′-end | 1 or 4 Wsa | 0.5 |
2 or 3 Wsa | 1.0 | |
3′-self-annealing or primer dimer | 5 nucleotides | 0.1 |
4 nucleotides | 0.2 | |
Less | 1.0 | |
Internal self-annealing or primer dimer | 8 nucleotides | 0.1 |
7 nucleotides | 0.3 | |
6 nucleotides | 0.8 | |
Less | 1.0 |