Table 3 Output format for best ranking primer pair
From: A tool for design of primers for microRNA-specific quantitative RT-qPCR
Name | Sequence | Score | Fprimer_anneal | Rprimer_anneal | Primer_dimer |
|---|---|---|---|---|---|
ssc -let-7a | tgaggtagtaggttgtatagtt | 0.32 | 1.0 | 1.0 | 1.0 |
F_1 | gcagtgaggtagtaggttgt | 0.64 | Â | Â | Â |
R_1 | ggtccagtttttttttttttttaactatac | 0.5 | Â | Â | Â |