Accuracy of estimated VAF in simulation. Plotted are the chances (Y axis) of the estimated VAF falling within 10% of the true VAF (X axis). Each data point is estimated from 1000 random samples. Each subplot in the figure contains 4 curves representing the accuracy of VAFs estimated from SNVs (red plus), 1 Kb deletions (green cross), 1 Mb deletions (blue triangle), and inversions/reciprocal-translocations (purple circle). Various types of sequencing data are simulated and results compared: a) short-insert (500 bp) short-read (100 bp) at 5× sequence coverage, b) short insert short read at 30× coverage, c) long insert (3000 bp) short read at 30x coverage and d) short insert short read at 500× coverage.