Figure 3

Several heatmaps of aligned transcript-pairs can be combined to provide a visual snapshot of sequence conservation. (A) Heatmaps for each pair-wise analysis of the human transcript encoding protein tyrosine phosphatase type IVA, member 1 (Ptp4a1) (NM_003463) with the othologous non-human transcript are shown. Black lines above each heatmap mark the position of the conserved uPEP and CDS for that species. Note the conservation of this uPEP even as the phylogenetic distance between the comparison species (on the right) widens. (B) ClustalW alignment of the Ptp4a1 uPEP, translated in silico from the conserved uORF. The numbers below the bar graph represent the conservation of each individual amino acid, where 10 (shown as an asterisk (*)) indicates identity across all species.