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Table 2 Comparison of the analyzed transcripts to RefSeq

From: spliceR: an R package for classification of alternative splicing and prediction of coding potential from RNA-seq data

 

WT GTF

Usp49KD GTF

 

Sensitivity

Specificity

Sensitivity

Specificity

Exon level

97.90

89.20

98.00

88.70

Intron level

96.40

97.00

96.40

96.90

Intron chain level

91.70

81.90

91.80

81.90

  1. Cufflinks’ cuffcompare tool (v. 2.1.1.) was used to compare RefSeq to the obtained transcripts and the sensitivity and specificity (as defined [20]) for exons, introns, and intron chains are shown.