Comparison TSSAR and TSSpredator . To assess the performance of TSSAR and TSSpredator we used dRNA-seq data of S. maltophilia. Thereby, the enrichment of cis-regulatory DNA motifs upstream of the predicted TSS was used as a surrogate for sensitivity. Furthermore, the individual results were compared to a manual annotation. Panel A shows the significantly enriched sequence motifs. Panel B shows the relative enrichment and the total count of this motifs in the sets of all TSS predicted by TSSAR, TSSpredator and by a manual analysis. Panel C depicts the overlap of the TSS annotated by the different methods.