Density plots with the average cutoff estimates. The averaged cutoff estimates of DAFS, Mclust, Sen0.85, Sen0.90, and Sen0.95 for simulated (a) log2 raw counts and (b) log2 RPKM data were presented. The black lines are empirical densities of the data. The black vertical lines present the DAFS cutoff estimates. The purple long-dashed lines, red dashed lines, blue dotted lines, and magenta dot-dashed lines present cutoffs estimated by Mclust, 85% sensitivity, 90% sensitivity, and 95% sensitivity, respectively.