Overview of the two discussed multiplexing technologies used for gene-drug and gene-gene interaction studies. (A) Luminex multiplexing for a gene-drug interaction screen. The gene perturbation is multiplexed and encoded as a DNA barcode that can be hybridized to Luminex beads and quantified. Drugs are not multiplexed. (B) MPS double multiplexing screen for gene-gene interactions. The first (genetic) perturbation can be multiplexed as a Illumina index barcode, and the second perturbation is identified by sequencing the hairpin cassette, which acts as a second DNA barcode.