Figure 6

Validation of the KRAS G13D Hotspot Mutation via IGV Automation. IGV automation is utilized to validate the non-synonymous hotspot mutation involving codon 13 for KRAS, where Glycine is replaced by Aspartic Acid (G13D). By a single point and click on the web page entry for KRAS, the IGV display is rendered. During this process, all relevant information is spontaneously sent to IGV. This includes data from Variant Analysis, RNA-seq aligned reads, WES aligned reads, the reference genome and RABT assembly. For illustrative purposes, the significant isoform, ENST00000311936, is circled in green, and the location of the G13D hotspot mutation is circled in red. A potential novel isoform (CUFF.19733.1) from the normal pool is circled in blue.