Figure 5

MS/MS spectra of the peptide DEHLSTLDApYRPK. This is a demonstrating example of the iPhos-facilitated phosphopeptide investigation. (a) From iPhos Module-1 and Module-2, the monoisotopic peak with m/z value of 812.858 was selected for targeted LC-MS/MS analysis. (b) The MS/MS spectrum of the identified pTyr peptide DEHLSTLDApYRPK. This peptide was identified to be the fragment of the protein "Similar to ankyrin repeat domain 20A, isoform CRA_a" (m/z 812.86, 2+) with a Mascot score of 39.