Shape parameters derived from segmented muscles can help quantify differences in atrophy patterns. The top chart compares the changes in the shape feature elongation between Atg9 and control RNAi expressing muscles. The ROIs used for the analysis were the same as in Figure 6a. In the first 25 hours AHE, both genotypes showed similar increases of elongation coinciding with muscle atrophy. In the next 70 hours, median elongation was significantly smaller in Atg9 compared to control muscles in almost all measurements, suggesting an inhibition of atrophy.