Components of the Janus Immunogenicity Score. JIS combines a prediction of MHC binding with a prediction of TCR binding conditioned on MHC binding. (left) MHC binding is predicted by EpiMatrix, which makes an allele-specific assessment of binding relative to a Normal distribution. The JIS contribution is the cumulative probability for those peptides exceeding a given threshold. (right) MHC-conditioned TCR binding is analyzed by JanusMatrix, which makes an allele-specific assessment of how many peptides in the human genome are predicted to bind the same MHC (possibly with different amino acids in the MHC pockets) and present the same amino acids to the TCR. The JIS contribution is the tail probability with respect to this empirical distribution. The plot is truncated at 10.