Performance comparison of Astroid with 3 different compression parameters (0 bp, 30 bp and 50 bp), Cufflinks, IsoLasso, Scripture and Trinity on 30M 2 × 75 bp (insert size around 250 bp) paired-end dataset. Evaluation measurements were plotted against different gene expression quantile (in 10% increments). (a) Each point in the plot represents the sensitivity of one method which is the ratio between the number of matched transcripts and the reference transcripts within one quantile. (b) Each point represents the precision of one method which is the number of matched transcripts and the total assembled transcripts within one quantile. (c) The correlation of transcript expression is computed on the set of matched transcripts for each method.