Coloured encoding of functional gene annotation. When a gene is present in a pan gene group it is represented by a unicoloured glyph. This glyph can be coloured based on TIGRFAM annotation, which can help to identify orthologous relationships, such as those that are highlighted. Though there are in principle hundreds to thousands of different TIGRFAM annotations, we restricted the colour encoding to 20 different colours chosen equidistantly from the HSB spectrum. Thus, a repetition of the colours may occur.