Figure 6

Parameter optimisation for colour deconvolution of a histology image (see case study). (a) The original H&E stained image of a liver section. (b) Deconvolution result of the hematoxylin stain using default values. (c) Deconvolution result of the eosin stain using default values. (d) Deconvolution result of the hematoxylin stain using more optimal values found using our method. (e) Deconvolution result of the eosin stain using more optimal values. Note that the aim is not feature detection but accurate isolation of individual dyes, which have overlapping absorption rates in different sub-cellular structures. Results (d) and (e) reflect the absorption rates of the component stains more accurately than (b) and (c).