Table 2 Statistics of contigs, Sanger sequences, and their alignments.
(a) Contig lengths of four known bacteria in three test samples. | ||||
|---|---|---|---|---|
Sample | Length of longest contig (bp) | |||
| Â | Legionella | Chryseobacterium | Pseudomonas | Bacillus |
S1 | 780 | 1483 | 1468 | 479 |
S2 | 1143 | 1052 | 1193 | N.A. |
S3 | 1487 | N.A. | N.A. | 845 |
(b) Results of BLAST alignments between each contig and the Sanger sequence. | ||||
Genus | Sanger (bp) | Identity (%); mismatch; gap | ||
| Â | Â | S1 | S2 | S3 |
Legionella | 832 | 99.8; 0; 1 | 99.6; 0; 2 | 99.6; 0; 2 |
Chryseobacterium | 1126 | 100; 0; 0 | 100; 0; 0 | N.A. |
Psuedomonas | 1529 | 100; 0; 0 | 99.8; 1; 1 | N.A. |