Figure 1

Computational methods revealed drug effects on protein translation. A Histogram of the ratio of TE in control and drug-treated samples. Red: genes with significant TE down and up regulation were identified based on the read count of Ribo-seq data. Blue and green: TE up and down genes defined by |Z score| > 2 as often used in other analyses. B Averaged distribution profile of ribosome occupancy of 62 drug-sensitive genes. Ribosome footprint coverages and transcript lengths were normalized. C Twelve-nucleotide motif that is highly enriched in 5' UTR of TE down and ROD positive genes. We suggested that the GQ structure is responsible for ribosome stalling in the 5' UTR [2].