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Figure 2 | BMC Bioinformatics

Figure 2

From: Alternative splicing detection workflow needs a careful combination of sample prep and bioinformatics analysis

Figure 2

Coverage and FPKM of splice variants in tss_total. A) Coverage distributions. The left-most boxplot refers to the coverage observed in ts100 for the set of splice variants that were not detectable in tss_total. The central (tss_total) and the right-most boxplot (ts100) report the coverage distribution for the splice variants that have been detected in both tss_total and ts100. B) As for A, but with respect to FPKM distributions. The left-most boxplot in panel A and B indicate that splice variants undetectable in tss_total are characterized by expression lower than that observable for the subset of the splice variants detectable by both tss_total and ts100. These low expression splice variants were not sampled in tss-total because the number of reads mapping to single copy genes were lower compared to other experiments. FPKM and coverage were calculated at transcript level using Cufflink (2.2.0)

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