Figure 7

Differential expression of the spike-in data. A) Synthetic spikes-in characterized by uniform coverage over the transcripts. B) TruSeq spikes-in. C) NuGEN spikes-in. Y-axes represent average log₂ fold change between T1-T5 and C1-C5 and X-axes represents average log₁₀ counts for the union of T1-T5 and C1-C5. Panel B highlights the homogeneous behaviors of TrueSeq spikes-in with respect to the number of reads used to generate the spikes-in. The differential expression of the spikes-in progressively increases from 20M to 80M reads. The same homogeneous behaviors cannot be detected in NuGEN (panel C)