Mapping the HMM histogram to the crystal structure of epithelial cadherin. A) The HMM histogram mapped onto the crystal structure (PDB code: 1EDH) of the first and second cadherin repeats of epithelial cadherin. Ca2+ ions are depicted as yellow spheres. The regions of high occurrence map to the Ca2+ binding site (blue represents low occurrence and red represents high occurrence)B) The HMM histogram of the epithelial cadherin subfamily with an e-value cutoff of 0.1. The orange bars in the histogram reflect positions involved in Ca2+ binding. Below the histogram is the domain layout. The features are colored: cadherin repeat (CR), blue rectangle; cytoplasmic domain (Cyt), green rectangle; catenin binding sites in the cytoplasmic domain, pink rectangles. The segment between the last CR and the cytoplasmic domain is the single pass transmembrane domain.C) The MSA of the segment involved in Ca2+ binding between the first and second CRs. The SWISS PROT code of the sequence is shown in the left and the 8 residues involved in Ca2+ binding are highlighted orange.