Figure 3

a). A portion of a scanned cDNA microarray showing the differential expression of PAC-1. The cDNA fragment spotted on position E6 corresponds to PAC-1. (I) Hybridization pattern for LGL patient. (II). Hybridization pattern for control. b). Shows the differential expression of PAC-1 in oligonucleotide array. Calculated fold change is 1.6 (I) Hybridization pattern for the PAC-1 probe set (RNA isolated from normal PBMC. PM = Perfect Match, MM = Mismatch. (II) Hybridization pattern for the PAC-1 probe set (RNA isolated from leukemic LGL cells. PM = Perfect Match, MM = Mismatch. c). Northern blot showing the expression of PAC-1 like genes. The probe used was the same as the one spotted on the microarray. Lane LGL = total RNA isolated from LGL leukemia patients (LGL). Lane N = total RNA isolated from normal healthy controls.