Figure 1
From: Development and production of an oligonucleotide MuscleChip: use for validation of ambiguous ESTs
Validation of MuscleChip by comparison to shared probe sets on HG-U95A stock chip, and the chip to chip variability of custom probe sets on the MuscleChip. Panel A. Shown is correlation of absolute analysis values (Avg Diff) for probe sets shared by the two chips, after hybridization with the same human normal muscle cRNA. There is an excellent correlation coefficient (0.89) between the two chips, although there is evidence of saturation of the HG-U95 chip for more abundant cRNAs. Panel B. Shown is the same data as in Panel A, but with removal of potentially saturated probe sets, and after adjustment for different scaling factors (MuscleChip Avg Diff times 3.18). A correlation coefficient of 0.89 is observed.