- ^ corresponds to the PROSITE motif 1 for the APE family, [APE]D [LIVMF]2x [LIVM]QExK * motifs correspond to PROSITE motif 2, D [ST] [FY]R [KH]-x(7,8) [FYW] [ST] [FYW]2 ** corresponds to PROSITE motif 3, NxGxR [LIVM]D [LIVMFYH]x [LV]xS. Completely conserved residues are bold, underlined letters are conserved to >70%. The first column shows the sequence of huApe1 with the MASIA consensus motif (50% conservation over 37 sequences) below it. The third column is the backbone RMSD between the indicated motif sequences in 1BIX (huAPE1) and the1AKO (E. coli ExoIII) crystal structures (both files are for the respective protein without DNA), with the number of atoms indicated in parentheses. The last column shows the effect of mutations in the motifs on APE activity and DNA binding.
