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Table 3 Dependent measure accuracies (i.e. comparison of test to reference alignment) for AMPS run on the Master data set at various gap-penalties sorted by Acc SCR – Clustering was performed on SD score (from 100 randomisations) with the PAM250 matrix. Acc SCR : Accuracy of Structurally Conserved Regions. AC a : Accuracy average (pairwise). AC w : Accuracy of whole alignment. PSE : Position Shift Error. NA in columns marked p indicates the highest accuracy in the preceding column, p gives the Wilcoxon Signed Rank test probability that the difference to the highest scoring row occured by chance.

From: OXBench: A benchmark for evaluation of protein multiple sequence alignment accuracy

Gap-penalty

Acc SCR

p

AC a

p

AC w

p

PSE

p

9

88.60

NA

82.30

0.1

74.29

0.09082

0.99

0.8841

12

88.48

0.46

82.49

NA

74.52

NA

0.96

NA

6

88.31

0.032

81.77

6.3e-05

73.64

7.076e-07

1.02

0.9658

15

88.08

0.031

82.31

0.13

74.34

0.2743

0.97

0.0956

3

87.47

5.7e-09

80.39

<2.2e-16

71.82

<2.2e-16

0.98

0.1704

18

87.44

9.0e-05

81.89

0.00020

73.82

0.0002315

1.01

0.000374

21

87.06

5.6e-07

81.68

4.2e-06

73.49

2.507e-07

1.02

4.4e-06

24

86.53

1.8e-10

81.30

5.5e-10

73.00

1.972e-10

1.04

7.565e-08

27

86.02

4.9e-13

80.97

7.6e-12

72.58

1.132e-13

1.12

5.164e-12

30

85.40

<2.2e-16

80.50

6.4e-16

71.85

<2.2e-16

1.17

1.94e-13

0

84.71

<2.2e-16

77.16

<2.2e-16

67.40

<2.2e-16

1.04

1.103e-13