Figure 3
Molecular architecture of CELO ORFs and selected homologs. (A) The red bar in ORF-2, ORF-12 and ORF-13 denotes homologous regions to the parvovirus NS-1 domain (Pfam PF01057). These domains are subdivided in a inactive ATPase/helicase domain of the helicase superfamily III and a region with no clearly defined function. CC: potential coiled-coil segments as reported by "COILS". Zn: region with four conserved cysteines in ORF-12 and ORF-13. ORF-13 has an extended and presumably unstructured amino-terminus rich in prolines and charged residues. In ORF-14, a distant homology to the superfamily III helicases could be detected in the region of the "Motif A" (see text). (B) Putative type-1 transmembrane glycoproteins. SP: signal peptides predicted by SignalP. TM: transmembrane domains predicted by TMHMM. In ORF-11, the TM is not significantly predicted. LCR: low complexity regions reported by SEG with parameters 25, 3.0, 3.3. The red bar denotes homology to the immunoglobulin-like domain (SMART SM00409). Potential Asn-glycosylation sites (PROSITE PS00001) could be found in all three ORFs: ORF-9 (41, 89, 114, 135, 181), ORF-10 (75, 92, 121, 157, 179, 198, 223, 229), ORF-11 (74, 84, 89). (C) The red bar indicates homology in ORF-16 to a family of vertebrate mono-ADP-ribosyltransferases (Pfam PF01129) as reported by CD-Search. (D) CELO-ORF-18/19 and homologous sequences in FAdV-9, FAdV-10 and Gallid herpesvirus 2 (NP_057756, a representative of Marek-disease like viruses). All have a lipase domain (Pfam PF00151) and a region unique to this group of avian viruses. The domain boundaries were estimated according to the location of PSI-BLAST hits to known lipases. "S/T" and "T" denote regions rich in serine/threonine and threonine, respectively. These domains are predicted to be highly O-glycosylated. In FAdV-10-ORF6050-7456 the blue colored region indicates a region of similarity to herpes glycoprotein D (Pfam PF01537) as reported by CD-Search. (E) Conserved transmembrane domains in CELO-ORF32895-32434 and its homologs in FAdV-9 and CFA40 predicted by TMHMM.