ΔΔG vs. Structural Variation for Pairwise Comparisons from the "Suboptimal Population". A set of 750 structure predictions (optimal + top 749 suboptimal) are compared, resulting in a total of 280,875 pairwise comparisons. The ΔΔG (pre efn2 re-evaluation) for two structure predictions is calculated by taking the absolute value of the difference between the ΔG of each structure prediction before efn2 re-evaluation. Structural variation for two structure predictions is calculated by counting the number of nucleotides in each structure prediction that either 1) have different pairing partners or 2) are paired in one structure prediction and unpaired in the other structure prediction (see Suboptimal Structural Variation Score in Methods). The shading within the figure indicates the number of pairwise comparisons that have the same values for both ΔΔG and structural variation score. A: Archaea 16S rRNA Haloferax volcanii. B: Archaea 16S rRNA Methanospirillum Hungatei.