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Table 2 Effects of different scoring criteria on CDS identification in the genome of S. typhimurium using IdentiCS and the database SWISS-PROT and TrEMBL. Criteria 1: E-value < = E-10; Criteria 2: E-value < = E-10 and Bits score > = 75; Criteria 3: E-value < = E-10, Bits score > = 75 and Identities > = 25%

From: IdentiCS – Identification of coding sequence and in silico reconstruction of the metabolic network directly from unannotated low-coverage bacterial genome sequence

 

Criteria 1

Criteria 2

Criteria 3

True positive

4339

4305

4303

False positive

987

602

555

False negative

110

144

146

Sensitivity %

97.5

96.8

96.7

Specificity %

81.5

87.7

88.6