Table 1 Features measured by the automated system. List of the behavioral and morphological features. Detail algorithms to output these features are found in supplemental data. 144 statistical results (mean, maximum and minimum where applicable) of these features are output into Microsoft Access, while the values of these feature at each time when an image is grabbed are saved in Microsoft Excel.
From: An imaging system for standardized quantitative analysis of C. elegans behavior
Features | Comments | Catalog |
|---|---|---|
Area | Total area of worm | Body morphology |
BendingFrequency | Frequency of body bends | Wave form/speed |
CmptFactor | Compactness factor | Body posture |
ElgFactor | Elongation factor | Body posture |
EllMAxis | Best-fit ellipse, major axis | Body posture |
EllRatio | Best-fit ellipse, major axis/minor axis | Body posture |
EqvlEllRatio | Equivalent ellipse ratio | Body posture |
Fatness | Area/Length | Body morphology |
Flex | Maximum skeleton point angle difference | Wave form |
Foraging | Frequency of sideways (foraging) head movements | Specific behavior (feeding) |
Foraging angle | Angle of foraging movements | Specific behavior (feeding) |
Foraging distance | Distance moved by head during foraging | Specific behavior (feeding) |
FRE | Frequency of angle change between skeleton points | Wave form/speed |
GlbMvScope | Maximum distance moved from starting point | Global movement |
GlbSpd | Speed of the animal's centroid movement | Global movement |
HdTlRatio | Ratio of head to tail movement speed | Head movement |
Heywood | Heywood circularity factor | Body morphology |
Hydraulic | Hydraulic radius | Body morphology |
IXX | Inertia XX | Body morphology |
IXY | Inertia YY | Body morphology |
IYY | Inertia XY | Body morphology |
LclSpd | Local movement speed | Local speed |
Length | Distance from head to tail | Body morphology/posture |
LengthToPixel | Length/number of pixels in skeleton | Body posture |
Loop | Percentage of time worms coils their body | Specific behavior (coil) |
MaxIntercept | Max intercept | Body morphology |
MeanIntcptPpdcl | Mean perpendicular intercept | Body morphology |
MostPopularArea | Mode of area | Body morphology |
MostPopularSpeed | Mode of speed | Global speed |
PercentageMPArea | Frequency of occurrence of modal area | Body morphology |
PercentageMPSpeed | Frequency of occurrence of modal speed | Global speed |
Pushing | Local body movement speed/centroid speed | Locomotion wave efficiency |
RectBigSide | Minimum enclosing rectangle (MER) length | Body posture |
RectRatio | Minimum enclosing rectangle length/width ratio | Body posture |
Reversal | Percentage of time that a worm performs reversals | Specific behavior (escape) |
ReversalCount | Number of reversal sessions that a worm performs | Specific behavior (escape) |
ReversalDisAve | Average distance traveled backward in reversals | Specific behavior (escape) |
SktAglAve | Mean value of skeleton point vector angle | Body bending |
SktCmptFactor | Length of worm / oriented MER area | Body posture |
SktElgFactor | Skeleton enlongation factor | Body posture |
SktHight | Oriented MER height | Body posture |
SktIXX | Sum of x coordinate times x coordinates of skeleton points | Body posture |
SktIXY | Sum of x coordinate times y coordinates of skeleton points | Body posture |
SktIYY | Sum of y coordinate times y coordinates of skeleton points | Body posture |
SktvAglAve | Average angle between skeleton points and centroid | Body bending |
SktvAglMax | Max angle between skeleton points and centroid | Body bending |
SktvDisAveToLength | Average distance between skeleton points and centroid/length | Body bending |
SktvDisMaxToLength | Max distance between skeleton points and centroid/length | Body bending |
SktvDisMinToLength | Min distance between skeleton points and centroid/length | Body bending |
SktWidth | Oriented MER width | Body posture |
Theta | Direction of centroid movement | Global movement |
Thickness | Thickness of worm | Body morphology |
TotalTravelDistance | Total distance traveled by a worm | Global movement |
TrackAmplitude | Amplitude of waves in worm's track | Wave form |
TrackWavlength | Wavelength of waves in worm's track | Wave form |
Transparency | Transparency of worm body | Body morphology |
Turn | Percentage of time that a worm performs a sharp turn | Specific behavior (search) |
TypeFactor | Type Factor | Body morphology |
Waddel | Waddel disk diameter | Body morphology |
WaveLenth | Largest spatial span of a sine wave | Wave form |
XSym | Sum of X coordinates of all sktps | Body bending |
XYSym | Sum f Y coordinates of all sktps | Body bending |
YSym | Sum of X coordinate * absolute value of Y coordinate | Body bending |