A general layout of microarray. In this example, a 2 × 2 pin configuration for 192 samples and 384 spots (duplicate) were considered. The origin is marked. Each pin prints one grid. One Super grid comprises of 4 grids, two in each axis. The number of super grids in X-direction is 2 and the number of super grids in Y-direction is 1. The spot pattern of a portion of one grid is zoomed for clarity. Replicates are spotted adjacent to each other in Y-direction. The number of samples in each grid is 24 and the total number of spots is 48. Two spots are further zoomed to show the diameter of the spot and the inter-spot distance.