Effect of positive variation normalization. A – Increasing rank differences in the comparison Wt-t0b vs Wt-t0a are plotted (in red) against the rank of Wt-t0a. Similarly, increasing rank differences in the comparison Wt-t0a vs Wt-t0b are plotted (in blue) against the rank of Wt-t0b. In the nomenclature of the TOR experiment, Wt and Mu refer, respectively, to strains SH100 and SH121, t0, t1 and t2 refer to 0 h, 2 h and 6 h time points, and a and b indicate the replicates. A sliding window of 100 points was moved by steps of 30 points. At each position, the mean (upper magenta curve) and std (lower green curve) were plotted and the resulting jagged lines were smoothed by a fitting procedure . The vertical arrows indicate the position of 10, 100 and 1000 signal on the 0–100 rank scale. B – Distribution of zRD (mean (magenta curve) and std (green curve) are not smoothed).