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Figure 1 | BMC Bioinformatics

Figure 1

From: Calibration and assessment of channel-specific biases in microarray data with extended dynamical range

Figure 1

Affine relationship between quantified fluorescent intensities and concentration of fluorophores. Scanning the arrays at different PMT gains indicates that there is an affine relationship between quantified fluorescent intensities and concentration of fluorophores. Left: Observed signals in the green channel for PMT pairs (800, 500), (700, 500), (800, 600), (600, 500), (700, 600), and (800, 700) are shown in green, red, cyan, black, blue and magenta, respectively. An affine model is estimated for each pair and displayed as a line. Data points at the very top are saturated and ignored. Right: A zoom-in of the same data. For each pair the estimated , which corresponds to the true origin, has been highlighted with a circle. All lines seem to intersect at the same point on the line. Shown are signals from the green channel on Array A quantified by GenePix from Axon scanner images.

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