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Table 1 Pairwise parameter estimates. Red and green channel estimates of the bias and the slope for each PMT voltage pair together with the mean and the standard deviation of all estimates. The small standard deviation is evidence that the bias to a large extent is independent of the PMT settings. Data shown originates from Array A scanned on Axon and analyzed with GenePix.

From: Calibration and assessment of channel-specific biases in microarray data with extended dynamical range

PMT pair

(800, 500)

18.9

32.4

20.7

32.5

(700, 500)

19.0

13.0

20.9

12.8

(800, 600)

17.3

7.1

19.8

7.9

(600, 500)

19.0

4.5

20.8

4.1

(700, 600)

17.6

2.9

20.9

3.1

(800, 700)

16.3

2.5

18.9

2.6

mean

18.0 ± 1.12

 

20.3 ± 0.80