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Figure 9 | BMC Bioinformatics

Figure 9

From: Biochemical Network Stochastic Simulator (BioNetS): software for stochastic modeling of biochemical networks

Figure 9

Schematic of the O R O lac engineered promoter. The promoter fuses three operator sites, one of which (O lac ) yields repression when the LacI tetramer is bound to it, while binding of the CI dimer to O R 2 yields approximately ten-fold activation (the O R 1 site assists activation by cooperatively enhancing CI binding to O R 2 when CI is bound to O R 1). In the experimental system, CI and LacI are produced in the cell by external promoters, not shown here. The Green Fluorescent Protein (GFP) product is then used to monitor the output of the promoter by flow cytometry.

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