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Table 2 Example of consolidation of BLAST output data for glutamine-hydrolyzing NAD(+) synthase.

From: A Bayesian method for identifying missing enzymes in predicted metabolic pathway databases

Sequences producing significant alignments

 

E-value

rank in output

fraction aligned

Hits found by Query Isozyme Q9CBZ6

    

CC3619 NAD(+) synthetase, putative

 

0.0

1

0.99

CC2963 hydrolase, carbon-nitrogen family

 

0.010

2

0.06

CC3013 TonB-dependent receptor

 

0.34

3

0.07

Hits found by Query Isozyme Q58747

    

CC3619 NAD(+) synthetase, putative

 

3e-07

1

0.70

CC1620 GMP synthase

 

0.029

2

0.14

CC0705 ORF

 

0.15

3

0.34

Hits found by Query Isozyme F86762

    

CC3460 ORF

 

0.024

1

0.43

CC3619 NAD(+) synthetase, putative

 

0.092

2

0.48

CC1541 2-isopropylmalate synthase

 

0.80

3

0.16

Hits found by Query Isozyme P18843

    

CC3619 NAD(+) synthetase, putative

 

2e-04

1

0.49

CC1541 2-isopropylmalate synthase

 

0.36

2

0.43

Hits found by Query Isozyme P47623

    

CC3619 NAD(+) synthetase, putative

 

6e-05

1

0.55

CC1620 GMP synthase

 

0.24

2

0.09

Results of data consolidation

consolidated data for hits from target genome

shotgun-score

best-E-value

average-rank

average-fraction-aligned

CC3619

5

0.0

1.2

0.64

CC1620

2

0.029

2

0.12

CC1541

2

0.36

2

0.30

CC3460

1

0.024

1

0.43

CC2963

1

0.010

2

0.06

CC3013

1

0.34

3

0.07

CC0705

1

0.15

3

0.34