Figure 1

Representative multiple alignment of the SPOC domain. The colouring scheme indicates average BLOSUM62 score (correlated to amino acid conservation) in each alignment column: cyan (greater than 3), light red (between 3 and 1.5) and light green (between 1.5 and 0.5). The limits of the domains are indicated by the residue positions on each side. The limits of proteins from partially sequenced genes whose full-length proteins are not available are not shown. X-Ray determined structure of the SPOC domain [9], pdb-code: 1OW1 is shown below the SHARP sequence (Swissprot-ID:MINT_HUMAN). PHD secondary structure prediction [29] for DIO family is shown below the DIO-1 human sequence (Swissprot-Id: DAT1_HUMAN), with E indicating a β strand (in red) and H an α helix (in green). The asterisks below the alignment marks the conserved pair arginine-tyrosine mentioned in the text. The sequences are named with their swissprot or sptrembl identifications, and also, if necessary, with their common species name: Human, Homo sapiens; Frog, Xenopus laevis; Drome, Drosophila melanogaster; Caeel, Caenorhabditis elegans; Arath,Arabidopsis thaliana; Ciona, Ciona intestinalis; Yeast, Saccharomyces cerevisiae; Fish, Brachydanio rerio; Plafal, Plasmodium falciparum; Aspnidu, Aspergillus nidulans; Pinus, Pinus taeda; Glycine, Glycine max; Schpo, Schizosaccharomyces pombe; Triti, Triticum aestivum. The "est" prefix identifies consensus sequences manually reconstructed by assembling highly similar expressed sequence tags from identical species (conceptual translations). The "unf" prefix identifies sequences obtained from Genome BLAST server at NCBI [24]. Complementary information is accessible at: http://www.pdg.cnb.uam.es/SPOC.