Figure 1From: Detection of nuclei in 4D Nomarski DIC microscope images of early Caenorhabditis elegans embryos using local image entropy and object trackingOverview of 4D DIC microscope images of C. elegans embryo. (A) Schematic of 4D DIC microscope images. Digital images of a developing embryo were recorded in multiple focal planes and a set of multifocal images was recorded with a fixed time interval, α. (B) Example of 4D DIC microscope images of a C. elegans embryo. Each column shows multifocal images recorded at a specific time point, with 3.5 μm between two focal planes. Each row shows time-lapse images recorded in a specific focal plane with 160 s between two time points. Bar is 10 μm.Back to article page