Table 4 Individual enzyme activities summed over all elementary modes Calculation of individual enzyme activities according to a given flux distribution: The 134 modes obtained from the input system [see Additional file 1] are all assumed to be active with standard (1 flux unit) activity. Alternatively, fractions of full activity of individual modes (given in percentages) can be set by the user and included in the calculation. For the standard flux vector, the total enzyme activities are calculated by YANA as follows (arbitrary units, only relative fluxes are calculated):
From: YANA – a software tool for analyzing flux modes, gene-expression and enzyme activities
Name | Act. (a.u.) | Name | Act. (a.u.) | Name | Act. (a.u.) | Name | Act. (a.u.) |
|---|---|---|---|---|---|---|---|
ALD | 203.0 | ApK | 111.0 | DPGM | 38.0 | EN | 598.0 |
GAPDH | 598.0 | GpoI | 209.0 | Gr | 399.0 | HYPXLeak | 74.0 |
LACex | 598.0 | PGI | 203.0 | PGK | 560.0 | PGLase | 576.0 |
PGM | 598.0 | Pmr | 201.0 | PNPase | 111.0 | PRM | 111.0 |
PRPPsyn | 111.0 | R5PI | 192.0 | TA | 192.0 | TKI | 192.0 |
TKII | 192.0 | TPI | 203.0 | TrxRI | 589.0 | Xu5PE | 384.0 |
ADA | 37.0 | AdPRT | 74.0 | AK | 38.0 | AMPase | 75.0 |
AMPDA | 37.0 | Cat | 6.0 | Cca | 37.0 | CgdI | 37.0 |
CgdII | 75.0 | CytI | 38.0 | DPGase | 38.0 | G6PD | 576.0 |
Gcl | 112.0 | GL6PDH | 576.0 | GLCim | 395.0 | Gls | 112.0 |
GtfI | 37.0 | GtfII | 37.0 | GtfIII | 38.0 | Gtr | 37.0 |
Har | 7.0 | HGPRT | 37.0 | HK | 395.0 | IMPase | 74.0 |
LDH | 598.0 | MemPhos | 38.0 | Nos | 196.0 | Opr | 38.0 |
Pdo | 99.0 | PFK | 203.0 | PK | 598.0 | Sod | 196.0 |
Tdi | 196.0 | Xen | 196.0 |