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Figure 2 | BMC Bioinformatics

Figure 2

From: Evaluating concentration estimation errors in ELISA microarray experiments

Figure 2

Spot fluorescence and standard concentration A graph of spot fluorescence as a function of standard concentration (A) shows that both the difference in concentration and the variability in spot fluorescence increase with concentration. Both negatively affect model fitting and subsequent statistical inferences due to the excessive leverage of the larger concentrations and the heteroskedasticity of the spot intensities. In this case, log e transforms of both concentration and spot fluorescence reduce the excessive leverage and improve the homogeneity (B). The log transformation is consistent with the featured concentration dilution series and is a common transformation of chemical measurements that often are assumed log normally distributed.

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