MPrime: efficient large scale multiple primer and oligonucleotide design for customized gene microarrays

BMC Bioinformatics

Table 1 Comparison of properties of MPrime with various primer and oligonucleotide design programs.

Program	Reference	Primers	Oligos	Large Scale Design	Sequence Similarity Avoidance
Primer Master	Proutski and Holmes (1996) [19]	X
PRIMO	Li, et al. (1997) [17]	X
GenomePRIDE	Haas, et al. (1998) [14]	X	X	X	X
Primer3	Rozen and Skaletsky (2000) [21]	X		X
DOPRIMER	Kampke et al., (2001) [15]	X		X
PrimeArray	Raddatz et al. (2001) [20]	X		X
GST-PRIME	Varotto et al. (2001) [23]	X		X
ProbeSelect	Li and Stormo (2001) [25]		X	X	X
PROBE	Pozhitkov and Tautz (2002) [27]		X	X	X
PRIMEGENS	Xu et al. (2002) [24]	X		X	X
OligoWiz	Nielsen et al. (2003) [26]		X	X	X
OligoArray 2.0	Rouillard et al. (2003) [29]		X	X	X
OligoPicker	Wang and Seed (2003) [30]		X	X	X
ROSO	Reymond, et al. (2004) [28]		X	X	X
MPrime	2005	X	X	X	X

ISSN: 1471-2105