Skip to main content

Table 2 Relative binding constants for six DNA binding sites for wild-type of zif268 and its 5 derivatives, where wild-type operator of zif268 was used as the reference. Each data were obtained from 5 or more independent examinations, inside of parenthesis are the standard deviations.

From: Quantitative analysis of EGR proteins binding to DNA: assessing additivity in both the binding site and the protein

DNA\Prot

RE(wt)

QE

DE

RN

QN

DN

CG(wt)

1

1

1

1

1

1

CA

0.27(0.06)

1.50(0.54)

1.16(0.49)

0.36(0.14)

0.49(0.19)

1.21(0.33)

CC

0.082(0.076)

2.17(0.91)

1.91(0.83)

0.41(0.23)

0.53(0.36)

2.61(0.59)

AG

0.15(0.10)

1.30(0.34)

1.48(0.56)

1.29(0.28)

4.45(2.64)

14.5(5.18)

AA

0.064(0.017)

1.36(0.48)

2.25(1.30)

0.68(0.28)

2.47(1.34)

4.02(1.56)

AC

0.041(0.045)

1.93(1.01)

3.08(0.45)

0.94(0.26)

2.78(0.80)

11.8(4.44)