Example of a PAI-like region and a cPAI in genome sequences. 48.5-kb of PAI ICFT073 from E. coli CFT073 was detected by merging genomic strips similar to known PAI loci (yellow strip) including partial sequence of PAI ICFT073. The genomic region contains homologs of the virulence genes on the known PAIs (red arrow) and genomic island (grey bar). Therefore, this PAI-like region is considered as a cPAI. Red and orange arrows in yellow strips denote virulence and putative virulence gene, respectively. Numbers on the yellow strips indicate parts of the PAI loci homologous to the genomic strips: 1. PAI I536 (accession number: AJ488511, host strain: E. coli 536); 2. PAI II536 (AJ494981, E. coli 536); 3. PAI III536 (X16664, E. coli 536); 4. LEE (AJ278144, E. coli 4797/97); 5 and 6. LEE (AF071034, E. coli O157:H7 EDL933); 7 and 8. PAI IICFT073 (AF447814, E. coli CFT073); 9. PAI ICFT073 (AF081284, E. coli CFT073); 10. PAI ICFT073 (AF081285, E. coli CFT073). Note that accessions of PAI IICFT073 that were included in the query set are partial sequence of the PAI. Some boxes are joined by a line for saving the space of the figure.