Figure 2

The main window of RATIO. Upon loading of MS data, analytes are first automatically recognized and assigned (A). When necessary, manual assignment of analytes is performed in a different window. The experimental parameters are set by the user (B), then MDV_M (C) and flux ratios (D) are calculated. Abnormal residuals indicate that the corresponding fragments are outlier, and they can be excluded (white) or reactivated (blue) by a single mouse click on the corresponding bar. Finally, the flux ratios, the MDV_A and the MDV_M are exported to a text file or Excel workbook (E).