Figure 3

Optimized between-group classification applied to tumour data. In panel A, 63 samples (solid circles) of the training set (BL: Burkitt's lymphoma, EWS: Ewing's sarcoma, NB: neuroblastoma, RMS: rhabdomyo sarcoma) and 25 samples (empty circles) of the test set (7, 15 and 18 as BL-NHL; 2, 6, 12, 19, 20 and 21 as EWS; 1, 8, 14, 16, 23 and 25 as NB; 4, 10, 17, 22 and 24 as RMS; 3, 5, 9, 11 and 13 as control samples that do not belong to one of the 4 groups) are classified by the standard BGA based on the whole set of genes. Panel B shows the different parameters of OBC as a function of the number of genes used in the analysis: the percentage of between group inertia (solid line), the percentage of good cross-validation (dashed line) and the variance of between group inertia (dot-dashed line). For indication, the percentage of test samples correctly predicted is represented by a dotted line. This parameter was not used in optimization of the training model. The vertical line shows the optimal number of genes. In panel C, the 245 most discriminating genes are represented with small crosses and the 90 optimal genes are highlighted (circled crosses). In panel D, the 25 test-samples are classified using a BGA based on the 90 optimal genes.