Figure 2
Filters on translation initiation for start-vertices and nonsense-mediated decay for stop-vertices. Circles: stop-vertices. Arrows: start-vertices. (1) Filters are not applied to vertices supported by protein-genome alignment (filled symbols). (2) Translation initiation filter: start-vertices preceded by at least two ATG codons in at least one path are removed. (3) Nonsense-mediated decay filter: stop-vertices for which the distance to the nearest donor site exceeds 55 nucleotides are removed.