Table 3 The main characteristics of the spots from three triplicates (see Fig. 5 (insets)), demonstrating excessive variability in the Cy5/Cy3 ratio estimates
From: An algorithm for automatic evaluation of the spot quality in two-color DNA microarray experiments
Triplicate | Quality Parameter | Spot 1 | Spot 2 | Spot 3 |
|---|---|---|---|---|
A | Ratio | 0.27 | 0.26 | 0.12 |
| Â | CD | 0.98 | 0.98 | 0.97 |
| Â | CVR | 0.016 | 0.014 | 0.11 |
| Â | IS | 0.17 | 0.21 | 1.02 |
| Â | UB | 0.060 | 0.10 | 0.31 |
B | Ratio | 0.19 | 0.13 | 0.072 |
| Â | CD | 0.85 | 0.60 | 0.33 |
| Â | CVR | 0.010 | 0.014 | 0.012 |
| Â | IS | 0.15 | 0.072 | 0.041 |
| Â | UB | 0.041 | 0.030 | 0.042 |
C | Ratio | 0.29 | 0.30 | 0.44 |
| Â | CD | 0.97 | 0.97 | 0.93 |
| Â | CVR | 0.010 | 0.011 | 0.0052 |
| Â | IS | 0.12 | 0.15 | 0.36 |
| Â | UB | 0.24 | 0.11 | 0.99 |