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Table 1 Test data used for the study of the phage communities with PHACCS [10-12]. The average fragment sequence length was determined using Sequencher after sequence trimming (maximum one ambiguity on 99 bp at each extremity). A 98% identity for a minimal overlap length of 20 bp was used for sequence assembly with Sequencher to obtain the contig spectra. The average genome length was determined by pulse field gel electrophoresis [12, 22].

From: PHACCS, an online tool for estimating the structure and diversity of uncultured viral communities using metagenomic information

Community

SP (Scripps Pier)

MB (Mission Bay)

MBSED (Mission Bay Sediments)

FEC (Fecal)

Contig spectrum *

1021 17 2 0 ...

841 13 2 0 ...

1152 2 0 ...

482 18 2 2 0 ...

Avg. community genome size

50 kb

50 kb

50 kb

30 kb

Avg. shotgun fragment length

663 bp

663 bp

570 bp

699 bp

  1. * The number of trailing zeros was set to 10 for each contig spectrum.